Regional Stream Quality Assessments



The Midwest Regional Stream Quality Assessment (MSQA)

A collaboration with the USEPA National Rivers and Streams Assessment (NRSA)

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Algae, benthic macroinvertebrates, and fish communities were sampled and physical habitat assessed once during summer 2013. Samples were collected along multiple transects within the stream reach. Basic water chemistry, including nutrient data, were collected at the same time as the biological data.

Water samples were collected weekly at each site for about 3 months preceding the ecological sampling, to capture the heavy agrochemical use and runoff season. Water samples were analyzed for nutrients, suspended sediment, sulfate, chloride, and about 230 dissolved pesticides and pesticide degradates.

Passive samplers were deployed in streams for about 7 weeks to collect dissolved chemicals from stream water and mimic the respiratory exposure of aquatic organisms. Two types of samplers were deployed, one that concentrates hydrophobic contaminants such as polycyclic aromatic hydrocarbons (PAH), and one that concentrates polar chemicals such as water-soluble pesticides and degradates.

Streambed sediment was sampled coincident with the ecological sampling. Surficial bed sediment was collected from depositional areas, composited, and sieved. All sediment samples were analyzed for about 25 trace elements and 120 currently used pesticides. At sites with urban land use in the basin, analyses also included polycyclic aromatic hydrocarbons (PAHs) and halogenated organic compounds (compounds, such as DDT, that contain chlorine or bromine atoms).

Ambient water samples were used to do 7-day toxicity tests to measure survival and reproduction of cladocerans (Ceriodaphnia) and survival and growth of fish (fathead minnow, Pimephales promelas). Bed-sediment samples were tested by means of a 28-day whole-sediment toxicity test with amphipods (Hyalella azteca) or freshwater mussels to measure effects on survival and growth.

Caged organisms (fathead minnows or leopard frogs, Lithobates species) were deployed at eight sites to test whether exposure to atrazine and other stressors in the field might cause effects on reproduction and organism health, similar to effects observed previously in laboratory exposures. Measured endpoints included egg production, growth metrics, histopathology, levels of steroid hormones and vitellogenin (an egg-related protein), and other biomarkers.

MSQA Sites
State Site Number Station Name Latitude Longitude
Illinois 03336645 MIDDLE FORK VERMILION RIVER ABOVE OAKWOOD, IL 40.136 -87.745
Illinois 03336890 SPOON RIVER NEAR ST. JOSEPH, IL 40.164 -88.027
Illinois 03384450 LUSK CREEK NEAR EDDYVILLE, IL 37.472 -88.547
Illinois 03611100 MASSAC CREEK NEAR MEtrOPOLIS, IL 37.169 -88.715
Illinois 03611200 MASSAC CREEK AT MEtrOPOLIS, IL 37.156 -88.708
Illinois 05531500 SALT CREEK AT WESTERN SPRINGS, IL 41.825 -87.9
Illinois 05550500 POPLAR CREEK AT ELGIN, IL 42.026 -88.255
Illinois 05554300 INDIAN CREEK NEAR FAIRBURY, IL 40.722 -88.53
Illinois 05572000 SANGAMON RIVER AT MONTICELLO, IL 40.03 -88.588
Illinois 05575550 CLEAR CREEK NEAR JEISYVILLE, IL 39.546 -89.435
Illinois 05592195 BECK CREEK AT HERRICK, IL 39.215 -89.02
Illinois 05599100 GALUM CREEK NEAR PYATTS, IL 37.944 -89.379
Illinois 384224087353601 ALLISON DITCH NEAR VINCENNES, IN 38.717 -87.594
Illinois 385730088324401 LITTLE WABASH RIVER NEAR MASON, IL 38.958 -88.545
Illinois 391136090341101 COLE CREEK NEAR HARDIN, IL 39.193 -90.569
Illinois 391601087414801 MILL CREEK NEAR CHOCTAW, IL 39.266 -87.696
Illinois 400856089562001 HERGET DRAINAGE DITCH NEAR KILBOURNE, IL 40.148 -89.938
Illinois 404917088222701 NORth FORK VERMILION RIVER NEAR WING 40.821 -88.374
Illinois 412911089540101 GREEN RIVER NEAR HOOPPOLE, IL 41.486 -89.9
Illinois 420626089101201 STILLMAN CREEK AT STILLMAN VALLEY, IL 42.107 -89.17
Indiana 03340900 BIG RACCOON CREEK AT FERNDALE, IN 39.711 -87.071
Indiana 03351072 WILLIAMS CREEK AT 96th StrEET, INDIANAPOLIS, IN 39.926 -86.172
Indiana 03352875 FALL CREEK AT 16th StrEET AT INDIANAPOLIS, IN 39.788 -86.177
Indiana 03353200 EAGLE CREEK AT ZIONSVILLE, IN 39.946 -86.26
Indiana 05517000 YELLOW RIVER AT KNOX, IN 41.302 -86.62
Indiana 384240087103901 PRAIRIE CR AT CO RD N100W NR CAPEHART, IN 38.709 -87.185
Indiana 390033085300301 OTTER CR AT N CORD 560E NR BUTLERVILLE, IN 39.009 -85.5
Indiana 391110087194401 W FK BUSSERON CR AT ST RT 48 NR WILFRED IN 39.186 -87.329
Indiana 391114085205801 OTTER CREEK AT W COUNTY RD 750N NR NAPOLEON, IN 39.184 -85.35
Indiana 392158086035901 NINEVEH CR AT STONE ARCH RD NR NINEVEH, IN 39.366 -86.066
Indiana 394253085111101 LICK CR AT N CORD 250W NEAR HARRISBURG, IN 39.714 -85.185
Indiana 394340085524601 SUGAR CREEK AT CO RD 400 S AT NEW PALESTINE, IN 39.727 -85.879
Indiana 401849087161401 BIG PINE CR AT CO RD N125E NR WILLIAMSPORT, IN 40.317 -87.291
Indiana 403242084553201 LIMBERLOST CREEK AT CORD N 250 E NEAR BRYANT, IN 40.545 -84.934
Indiana 411439087065601 HODGE DITCH StrEAM AT CORD N400W NR WHEATFIELD, IN 41.242 -87.121
Iowa 05418180 Johns Creek near Worthington, IA 42.397 -91.062
Iowa 05418400 North Fork Maquoketa River near Fulton, IA 42.164 -90.729
Iowa 05420520 Wapsipinicon River at McIntire, IA 43.443 -92.611
Iowa 05451112 South Fork Iowa River at H Avenue near Buckeye, IA 42.436 -93.346
Iowa 05451210 South Fork Iowa River NE of New Providence, IA 42.315 -93.152
Iowa 05455095 Old Mans Creek at Kansas Ave SW near Iowa City, IA 41.608 -91.638
Iowa 05457520 Cedar River at Lancer Avenue at Osage, IA 43.253 -92.812
Iowa 05458800 Maynes Creek near Hampton, IA 42.68 -93.203
Iowa 05464220 Wolf Creek near Dysart, IA 42.251 -92.298
Iowa 05471090 Unnamed Trib to East Br Indian Cr near Zearing, IA 42.106 -93.35
Iowa 05481820 Beaver Creek at Bouton, IA 41.849 -94.028
Iowa 05482300 North Raccoon River near Sac City, IA 42.354 -94.99
Iowa 05483341 Beaver Creek at Glendon, IA 41.589 -94.401
Iowa 05483450 Middle Raccoon River near Bayard, IA 41.779 -94.492
Iowa 05487550 Walnut Creek near Vandalia, IA 41.537 -93.258
Iowa 06808495 Unnamed Trib to W Nishnabotna Riv nr Randolph, IA 40.873 -95.594
Iowa 06897937 Long Creek at 137th Street near Van Wert, IA 40.838 -93.857
Kansas 06893350 TOMAHAWK C NR OVERLAND PARK, KS 38.906 -94.64
Kansas 393358096130000 FRENCH C AT PARALLEL RD, ONAGA, KS 39.566 -96.216
Kansas 394306095484300 MUDDY C AT 145th ST NR WETMORE, KS 39.718 -95.811
Kentucky 03383782 RICHLAND CREEK AT CARBONDALE RD NEAR RICHLAND, KY 37.266 -87.601
Kentucky 372629086330400 CANEY CREEK NEAR CANEYVILLE, KY 37.441 -86.551
Kentucky 373514086371200 POND RUN AT HIGHWAY 110 NEAR FALLS OF ROUGH, KY 37.585 -86.618
Minnesota 05299770 FLORIDA CREEK AT 171ST AVE NEAR MARIETTA, MN 44.902 -96.309
Minnesota 05315295 thREEMILE CREEK AT 210th AVE NEAR GHENT, MN 44.49 -95.877
Minnesota 0531656290 WEST FORK BEAVER CREEK AT 320 ST. NEAR BECHYN, MN 44.69 -95.035
Minnesota 05320410 MAPLE RIVER AT HWY 30 NEAR MAPLETON, MN 43.907 -94.04
Minnesota 05320500 LE SUEUR RIVER NEAR RAPIDAN, MN 44.109 -94.041
Minnesota 05325148 SEVENMILE CK BLW FOOTBRIDGE IN PARK NR KASOTA, MN 44.262 -94.029
Minnesota 05457200 CEDAR RIVER AT 100th ST. NEAR LYLE, MN 43.514 -93.002
Missouri 06893620 Rock Creek at Kentucky Road in Independence, MO 39.111 -94.472
Missouri 383158092192001 North Moreau Creek nr Jefferson City, MO 38.535 -92.318
Missouri 385638091364601 Loutre River near Montgomery City, MO 38.943 -91.612
Missouri 390200092341701 Moniteau Creek near Rocheport, MO 39.033 -92.571
Missouri 390227092234101 Perche Creek near Columbia, MO 39.041 -92.394
Missouri 391308091550901 Skull Lick Creek nr Mexico, MO 39.218 -91.919
Missouri 391443091534001 Fish Branch near Mexico, MO 39.245 -91.894
Missouri 391504093003301 Bear Creek nr Gilliam, MO 39.251 -93.009
Missouri 391815009203901 Goodwater Creek nr Centralia, MO 39.304 -92.052
Missouri 394151094531501 Contrary Creek nr St. Joseph, MO 39.697 -94.887
Missouri 394254094092301 Brushy Creek near Cameron, MO 39.715 -94.156
Missouri 400227095151501 Squaw Creek Ditch near Squaw Creek Wildlife Area 40.04 -95.254
Missouri 400240092081201 Little Fabius River nr Fabius, MO 40.044 -92.136
Missouri 400502091403401 North Fabius River nr Monticello, MO 40.083 -91.675
Nebraska 06610765 Little Papillion Cr at Ak-Sar-Ben at Omaha, Nebr. 41.245 -96.02
Nebraska 06610785 West Papillion Creek at Millard, Nebr. 41.207 -96.127
Nebraska 06800000 Maple Creek near Nickerson, Nebr. 41.561 -96.54
Nebraska 06804000 Wahoo Creek at Ithaca, Nebr. 41.147 -96.537
Nebraska 401143096134301 Turkey Creek near Steinauer, Nebr. 40.189 -96.223
Nebraska 413012096210001 Bell Creek near Arlington, Nebr. 41.493 -96.348
Nebraska 420444097543301 Elkhorn River near Oakdale, Nebr. 42.069 -97.933
Nebraska 424048097483601 Howe Creek near Lindy, Nebr. 42.695 -97.788
Ohio 03241500 Massies Creek at Wilberforce OH 39.722 -83.882
Ohio 03259000 Mill Creek at Carthage OH 39.201 -84.471
Ohio 03271000 Wolf Creek at Dayton OH 39.766 -84.236
Ohio 395942083151401 Big Darby Creek at Prairie Oaks nr Lake Darby, OH 39.995 -83.253
Ohio 410133082465301 Unnamed Trib to Honey Creek near Willard, OH 41.025 -82.781
Ohio 410150083125701 Sandusky River at CR6 near McClutchenville, OH 41.03 -83.215
Ohio 411146082244001 Vermilion River at SR18 near Clarksfield, OH 41.196 -82.411
South Dakota 435420096290500 PIPESTONE CREEK NR SD/MN STATE LINE 43.905 -96.484
Wisconsin 040852508 TISCH MILLS CREEK AT TISCH MILLS, WI 44.327 -87.636
Wisconsin 040857005 OTTER CREEK AT WILLOW ROAD NEAR PLYMOUth, WI 43.788 -87.921
Wisconsin 040869416 LINCOLN CREEK @ SHERMAN BOULEVARD AT MILWAUKEE, WI 43.097 -87.967
Wisconsin 04087119 HONEY CREEK AT WAUWATOSA, WI 43.043 -88.005
Wisconsin 05426400 SCUPPERNONG RIVER NEAR PALMYRA, WI 42.886 -88.541
Wisconsin 05544989 HONEY CREEK AT CT HIGHWAY DD NEAR BURLINGTON, WI 42.719 -88.309